Separation of Transfer Ribonucleic Acids by a Simple Extraction Process
نویسندگان
چکیده
منابع مشابه
Separation of transfer ribonucleic acids by reverse phase chromatography.
Numerous experimental techniques for the separation of transfer ribonucleic acids have been used successfully in preparing partially purified fractions of several specific tRNAs.1 Many of the existing methods have depended upon the differential solubility of specific tRNAs in complex two-phase systems (I-B), and the separations were achieved by means of countercurrent extraction techniques. Col...
متن کاملChromatographic separation of mononucleotides derived from transfer ribonucleic acids.
1. A method is described for fractionating alkaline and enzymic hydrolysates of ribonucleic acids and oligonucleotides on DEAE-cellulose columns by using a constant-composition eluent. 2. The relative partition coefficients of the four major and several minor nucleotides present in nucleic acid digests are given. 3. The influence of pH and of molarity of the buffer on the separation is describe...
متن کاملIsolation of "renaturable" transfer ribonucleic acids.
A new type of fractionating step involving gel Cltration is introduced for the purification of certain transfer ribonucleic acids (tRNAs). It is based on the increase that occurs in the molecular dimensions of these tRNAs when they are trapped, reversibly, in a denatured state under conditions where the other tRNA components of the mixture are in the native conformation. This step, coupled to c...
متن کاملIsolation of Specific Transfer Ribonucleic Acids
1. HAGELBERG, L., Ber. de&. hem. Ges., 23,1083 (1890). 2. KATJFMANN. H. P.. GINDSBERG. E.. ROTTIG, W.. AND SALCHOW, R., Ber. $eut. chkm. Ges., 70, 2519 (1937). ’ ’ 3. SIEGEL, J. R., AND ROSENBLATT, D. H., J. Am. Chem. Sot., 80, 1753 (1958). 4. GABRIEL, S., Ber. deut. them. Ges., 22, 1139 (1889). 5. FIESER, i. I?., Experiments in organic chemistry, Ed. 2, D. C. Heath and Co., New York, 1941, p. ...
متن کاملThe extraction and separation of bile acids.
As experience is gained in the attempt to apply counter-current distribution to the separation of complicated biological substances from the mixtures in which they naturally occur, it becomes abundantly clear that the solubility and the hydrophobic or hydrophilic properties of known pure components are markedly altered in such mixtures. Thus, one solute may carry with it into solution a second ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1965
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)97602-7